Read length distribution of PacBio

Question

[midox]

Hello, I need the distribution of reads length generated from PacBio sequencer. can i find an histogram,....? because i would like to create a file with reads similary to the distribution of long reads generated by PacBio (like number of reads, length of reads,...). Can you help me?

Thanks

[GenoMax]

You could get some real pacbio datasets and then calculate this yourself. PacBio makes a selection available here or you can also use SRA/ENA.

[midox]

no, I just want to get the distribution of reads . I want to create a sequence file ( long Reads ) very similar to the PacBio distribution. as if the long reads generated by the sequencer PacBio .

Answer 1

[Antonio R. Franco]

You can find this information in their own blog

But be aware that they change the chemistry and tune the DNA polymerase to improve this very rapidly

[midox]

thanks for the link. but in the first histogram. The number of all reads is the sum all bars of the histogram? thanks