Finding novel transcripts of an organism

Question

[Prakki Rama]

Hi all,

Could I please know if searching against whole NR and NT database would be sufficient to know if a sequence is novel or not? What other resources, do I need to check to find if it is a novel transcript? I would like to seek your kind advice and suggestions. Thanks in advance.

[Prakki Rama]

Seems like it has already been answered in a Biostar post.

[Farbod]

Dear Prakki Rama,

would you please have a look at this post, too : C: what is the prefered strategy to treat with blast-less transcriptomes from RNA-s

may be it depends that what is your species (model animal?) and is there any reference genome for it ?