I have bisulfite sequencing data that was aligned and provided in bigwig format. I've since converted the files to bedgraph format, and based off the naming of the files, the BS-seq data was split by +/- strand (i.e., sample1 has two bedgraph files, one bedgraph for the reads on the plus strand, and another bedgraph for reads on the negative strand). I noticed that the start and end coordinates on the plus strand sample to be 1 position lower than the negative strand samples. I appreciate any insight in explaining this discrepancy.
Thanks for the explanation.