Determining Up and Down regulated genes from GEO GDS4296
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8.1 years ago
hAjmal ▴ 50

Hi All, How to see if the genes are up or down regulated in the publicly available dataset GDS4296 which contains analysis from cell lines from 9 different cancer tissue of origin types (Breast, Central Nervous System, Colon, Leukemia, Melanoma, Non-Small Cell Lung, Ovarian, Prostate, and Renal) from the NCI-60 panel? Regards

gene Regulation R Geo • 2.5k views
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Have you tried the GEO2R option available from within GEO? That a quick and easy method that tends to work well enough.

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Yes I have looked into that, but what I dont understand is what will be the control(no cancer) group?

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Control is the group of samples, with which you will have to compare the patient samples. For each tissue, there should be different controls.

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Thats where I find it confusing. I want to analyze this GEO dataset with levels in non cancerous samples. I dont know which ones are the control (non cancer) samples, if any

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Can you mention the GEO ID?

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The acession number is GEO GDS4296

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The cancer panel doesn't have controls with it. You can only look at differences between cell types.

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Thanks. I have moved to another dataset "GSE62944"

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