Differential expression of miRNA

Question

[Tanvir Ahamed]

miRNA expression data has generated form both DNA and Exosome of same patient. Data structure is as follows.

Si = Same patient (i = 1,2 .....,10) [Here 10 patient]

Can anyone suggest any good statistical approach to analyse the data with an aim for differential expressed miRNA?

NB: I am feeling it is a nested design. But cant think any good statistical approach.

Thanks in advance

[Benn]

Check limma, there are many experimental designs presented in the manual. You'll probably want make a model which includes the paired samples as a factor, the normal/child/adult status, and the source (DNA or Exosome).

[Tanvir Ahamed]

I will check. Thanks.

Answer 1

[Tanvir Ahamed]

Any one has any idea or reference of article ?

[Benn]

Do you know how to use pubmed or google scholar? Pretty handy sometimes ;)

[Tanvir Ahamed]

Thanks though !! Looking for a viable solution ...you have shown me the sea :)