I have an Agilent dataset, I am trying to read and normalize it, I used the code bellow but I am getting error.
library(limma)
targets <- readTargets("targets_limma.txt", row.names=1,sep ="")
x <- read.maimages(targets$Filename,source="agilent",green.only=TRUE)
Can we use RMA tp normalize Agilent data?
NormData<-rma(data)
does not work.
Please any help