AUGUSTUS principal isoform

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9.3 years ago

jomaco ▴ 200

Hi,

I have used AUGUSTUS for gene prediction.

It produces multiple isoforms for some genes. These are listed as XXXX.1, XXXX.2, XXXX.3 etc.

Which isoform can be taken to be the principal isoform i.e. the isoform used in further analysis?

I ask as it seems most genome papers only report functional descriptions for a single isoform.

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However, I could report functional descriptions for all isoforms?

In lieu of this, at least for OrthoMCL clustering the selection of a principal isoform seems paramount (such that the output is not inundated with gene families consisting of matches between different isoforms of the same gene in the same species).

Until now I have simply gone with the isoform labelled ".1" - is this okay?

Thanks,
Jon.

isoform augustus principal-isoform • 2.4k views

ADD COMMENT • link updated 2.1 years ago by Ram 43k • written 9.3 years ago by jomaco ▴ 200

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Which species are you interested in?

ADD REPLY • link 9.3 years ago by Denise CS ★ 5.2k

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Don't think I'm supposed to say - but it's a species in the asterids if that helps, newly sequenced

ADD REPLY • link 9.3 years ago by jomaco ▴ 200

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I see. I was thinking of APPRIS currently run for human, mouse, zebrafish, rat and others. Not sure if it would be useful for your study case. You may want to contact the authors.

ADD REPLY • link 9.2 years ago by Denise CS ★ 5.2k

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