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116,023 results • Page
1 of 2321
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0
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Error running local blastn in R using system2
blastn
NCBI
R
updated 9 minutes ago by
GenoMax
140k • written 31 minutes ago by
Harrison
• 0
14
votes
8
replies
386
views
Forum:
F*up Night style events for Bioinformatics ? Comment if you're interested!
mistakes
updated 39 minutes ago by
Istvan Albert
100k • written 4 hours ago by
Ann
★ 2.4k
0
votes
0
replies
13
views
News:
Online course: Reproducibility in Bioinformatics
Singularity
Version-Control
Docker
Containers
updated 52 minutes ago by
Ram
43k • written 1 hour ago by
carlopecoraro2
★ 2.4k
0
votes
0
replies
14
views
News:
Online course: Analysis of Prokaryotic Pangenomes (Last call)
Prokaryotic
Bacterial-Genomics
Pangenomics
updated 50 minutes ago by
Ram
43k • written 1 hour ago by
carlopecoraro2
★ 2.4k
1
vote
9
replies
3.4k
views
Error in CIBERSORTx
RNA-seq
CIBERSORT
LM22
updated 1 hour ago by
Ram
43k • written 17 months ago by
Manav
• 0
0
votes
1
reply
76
views
Annotation of Complex genomes using NCBI Genome Workbench
Genome-Workbench
MAKER
updated 2 hours ago by
Lissa Cruz Saavedra
• 0 • written 16 hours ago by
SAsciences
• 0
0
votes
0
replies
23
views
Is RNAseq data from a monoclonal cell line technically considered scRNAseq?
scRNA
RNAseq
2 hours ago by
sithlordashley
• 0
0
votes
1
reply
31
views
Looking for RPIP Illumina kit probe sequences
Sequences
Illumina
updated 2 hours ago by
GenoMax
140k • written 3 hours ago by
David Tachiquín
• 0
0
votes
8
replies
92
views
Low number of both surviving reads after trimming
trimmomatic
trimming
updated 10 minutes ago by
GenoMax
140k • written 3 hours ago by
Jay
• 0
793
votes
167
replies
139k
views
109 follow
News:
The Biostar Handbook. A bioinformatics e-book for beginners.
training
handbook
updated 9 weeks ago by
Biostar
2.6k • written 7.3 years ago by
Istvan Albert
100k
0
votes
2
replies
98
views
ONT direct RNA sequencing
DRS
ONT
updated 1 hour ago by
Ram
43k • written 8 hours ago by
Karina
• 0
0
votes
1
reply
52
views
Number of CPUs to use by DRAP for transcriptomic assmbly
DRAP
CPU
transcriptomics
updated 2 hours ago by
GenoMax
140k • written 4 hours ago by
Mohamed Abderrahmane
▴ 10
2
votes
2
replies
67
views
Gene reads all zero for each sample
R
salmon
DE
DESeq2
4 hours ago by
DYLAN NICO
• 0
0
votes
2
replies
160
views
Can I have multiple active GDC download tokens?
TCGA
updated 5 hours ago by
Zhenyu Zhang
★ 1.1k • written 3 days ago by
mrk
• 0
0
votes
0
replies
41
views
Contradictions in GSEA results
GSEA
Enrichment-analysis
PEA
updated 5 hours ago by
Ram
43k • written 5 hours ago by
Tolga
▴ 20
0
votes
0
replies
36
views
Illumina Dna Prep kit for sequencing phage linear genomes
tagmentation
transposons
phagetermini
sequencing
5 hours ago by
alenew.am
• 0
0
votes
3
replies
251
views
FindAllMarkers not working (Error (data layers are not joined. Please run JoinLayersWarning: When testing 1 versus all)
FindAllMarkers
seurat
re-clustering
5 hours ago by
Nitin
• 0
0
votes
1
reply
70
views
(sc)RNA-seq dataset for these cell lines: HEK293, HeLa, PC3 and U2OS
scRNA-seq
updated 5 hours ago by
Ram
43k • written 6 hours ago by
chiditatawmbi
• 0
0
votes
1
reply
92
views
Normalization strategies for comparing mapped reads across samples in RNA-seq
normalization
rnaseq
updated 5 hours ago by
Ram
43k • written 6 hours ago by
Picasa
▴ 640
0
votes
0
replies
54
views
How to change alternate conformation occupancy ?
Bio.PDB.Atom
Bio.PDB
biopython
updated 4 hours ago by
Ram
43k • written 7 hours ago by
udakshi
• 0
0
votes
0
replies
72
views
Training
chip-seq
ScATAC-seq
7 hours ago by
Manhezz
• 0
1
vote
2
replies
112
views
Issue with making local BLAST database
R
mitochodrial
diatoms
blast
ncbi
updated 1 hour ago by
Ram
43k • written 8 hours ago by
Begonia_pavonina
▴ 140
1
vote
1
reply
268
views
diffbind : killed dba.count(DBsample, bParallel=FALSE)
diffbind
r
atacseq
8 hours ago by
Pierre Lindenbaum
160k
1
vote
2
replies
96
views
Flow Cytometry Data Analysis by Seurat
Seurat
updated 4 hours ago by
Ram
43k • written 8 hours ago by
gdfsnkfns
• 0
1
vote
11
replies
259
views
struggle to get fasta files from ucsc goldenPath
ucsc
getfasta
fasta
updated 3 hours ago by
GenoMax
140k • written 9 hours ago by
Lila M
★ 1.2k
2
votes
2
replies
93
views
Volcano Plot Output Inquiry: Graphs Facing Down
rstudio
volcano-plot
updated 4 hours ago by
dariober
14k • written 9 hours ago by
Pumla
• 0
0
votes
1
reply
75
views
Merging featureCounts output for different species
featureCounts
RNA-seq
updated 1 hour ago by
Ram
43k • written 10 hours ago by
Christian
• 0
2
votes
2
replies
116
views
Interproscan taking so much time
slurm
interproscan
transcriptomics
updated 10 hours ago by
dthorbur
★ 1.7k • written 10 hours ago by
Mohamed Abderrahmane
▴ 10
0
votes
0
replies
54
views
Combine rename and rmdup in SeqKit to remove duplicate sequences and append N in header? Sort by occurence?
seqkit
fasta
10 hours ago by
Broccoli
• 0
0
votes
0
replies
52
views
Is there a good tool/reference for PBP and porin analysis
PBP
porin
10 hours ago by
blur
▴ 280
0
votes
0
replies
59
views
meta-analysis on RNA seq AND microarray results
microarray
RNA
11 hours ago by
liuyibin2005
• 0
49
votes
30
replies
11k
views
13 follow
Tutorial:
How to Use Biostars, Part-I: Questions, Answers, Comments and Replies
documentation
meta
how-to
updated 7 hours ago by
Istvan Albert
100k • written 8.1 years ago by
Ram
43k
0
votes
0
replies
49
views
SPOTlight deconvolution logFC, logFC cohen or mean.AUC
spatial-transcriptomics
SPOTlight
logFC
deconvolution
updated 5 hours ago by
Ram
43k • written 11 hours ago by
M'zelle
• 0
0
votes
5
replies
217
views
calculate mismatch rate from VCF file
mismatch-rate
VCF
updated 5 hours ago by
Ram
43k • written 17 hours ago by
Dora
▴ 10
2
votes
4
replies
286
views
Hybrid assembly Pacbio- Nanopore
long-reads
hybric
assembly
genome
short-reads
2 hours ago by
Lissa Cruz Saavedra
• 0
0
votes
0
replies
67
views
In scRNA, How should I set QC standards?
scRNA
QC
14 hours ago by
mnx0723
• 0
0
votes
2
replies
233
views
Error occurs when migrate-n software performs "make mpis"
migrate-n
16 hours ago by
lingxue
• 0
0
votes
0
replies
68
views
How to check if a few pathways have relationship?
MSigdb
REACTOME
KEGG
18 hours ago by
Chris
▴ 260
2
votes
10
replies
705
views
Only one read per run - Trying to use CellRangerv7
Fastq
CellRanger
updated 5 hours ago by
Ram
43k • written 5 weeks ago by
Sky
▴ 10
3
votes
2
replies
276
views
FDR and Bonferroni
FDR
Bonferroni
updated 18 hours ago by
joe
▴ 470 • written 2 days ago by
song
• 0
0
votes
1
reply
478
views
Which fasta files are assembled transcripts IDBA_tran assembler ?
RNA-Seq
Assembly
updated 20 hours ago by
weidonglu
• 0 • written 3.8 years ago by
sunnykevin97
▴ 980
0
votes
0
replies
82
views
doing some ontology enrichment analysis
GO
21 hours ago by
aleksk779
• 0
2
votes
1
reply
178
views
Choosing an FC Threshold
statistics
rna-seq
updated 20 hours ago by
Gordon Smyth
★ 6.9k • written 1 day ago by
Netanel
• 0
0
votes
1
reply
115
views
Converting AutoDock-Vina PDBQTs to PDB files
Docking
AutoDock
Vina
PDBQT
PDB
updated 23 hours ago by
Mensur Dlakic
★ 26k • written 1 day ago by
Ricard
• 0
0
votes
2
replies
155
views
novel and know mir156 and mir172
a
updated 20 hours ago by
gayachit
▴ 200 • written 23 hours ago by
Fadmo
• 0
0
votes
1
reply
100
views
Update R in Posit Workbench
R
Posit
updated 1 day ago by
Ram
43k • written 1 day ago by
Nitin
• 0
2
votes
8
replies
251
views
Piping samtools to R
R
NGS
bash
samtools
1 day ago by
joe
▴ 470
8
votes
6
replies
390
views
Pangenome of plant
pangenome
plant
updated 1 day ago by
talbots
• 0 • written 2 days ago by
analyst
▴ 10
1
vote
2
replies
181
views
vg call is time consuming
vg
1 day ago by
Maxine
▴ 40
4
votes
14
replies
4.5k
views
8 follow
Installation: Tax4Fun2 package are not found and github repository is not maintained anymore
Tax4Fun2
updated 1 day ago by
Ram
43k • written 21 months ago by
Elsa
▴ 20
116,023 results • Page
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Comment: Error running local blastn in R using system2
by
GenoMax
140k
Do you have the file called `nt_euk.nal`? What do you see if you `cat nt_euk.nal`?
Comment: Low number of both surviving reads after trimming
by
GenoMax
140k
It is your data and if you want to do that it is up to you. You may be throwing away essentially good data (you should remove the nextera s…
Comment: F*up Night style events for Bioinformatics ? Comment if you're interested!
by
Istvan Albert
100k
Some things never change ... the most common mistake is still the one on top there
Comment: Low number of both surviving reads after trimming
by
Ram
43k
I see that the code content was not added again, just the formatting modified. The output is still mangled. Compare it to the output you se…
Answer: F*up Night style events for Bioinformatics ? Comment if you're interested!
by
Mensur Dlakic
★ 26k
I was trying to avoid re-living traumatic events, but here goes. This is an attempt to delete all the files with a certain extension but in…
Comment: Low number of both surviving reads after trimming
by
Jay
• 0
Thank you for your response. There was no particular reason of `LEADING:20 and TRAILING:20`, but I used the same parameters as I had used …
Comment: Low number of both surviving reads after trimming
by
GenoMax
140k
A significant fraction of your reads appear to have the nextera sequence, that combined to this directive `LEADING:20` (do you have a reaso…
Comment: Low number of both surviving reads after trimming
by
Jay
• 0
Here is my fastqc, especially adapter content graph, before trimming(R1 and R2): ![enter image description here][1] And this after trimm…
Comment: Low number of both surviving reads after trimming
by
Jay
• 0
Thanks, I modified.
Comment: F*up Night style events for Bioinformatics ? Comment if you're interested!
by
Pierre Lindenbaum
160k
11 years ago: https://www.biostars.org/p/7126/
Comment: F*up Night style events for Bioinformatics ? Comment if you're interested!
by
Dave Carlson
★ 1.7k
haha yes exactly! After the first or second ruined fasta, I started "catting" all fasta files and piping to other commands, just to reduce …
Comment: F*up Night style events for Bioinformatics ? Comment if you're interested!
by
Istvan Albert
100k
... ha, beat me to it, posted at the same time ... this error rules them all .. here is a cool trick on how to count fasta headers in file…
Answer: F*up Night style events for Bioinformatics ? Comment if you're interested!
by
Dave Carlson
★ 1.7k
I've definitely ruined some genome fasta files by running `grep ^> mygenome.fasta` instead of `grep "^>" mygenome.fasta`
Comment: Error in CIBERSORTx
by
o.nad
• 0
Hello. I faced the same problem but not able to resolve it. I don't have double quotation and no NA values and the dataset used is in the f…
Answer: Annotation of Complex genomes using NCBI Genome Workbench
by
Lissa Cruz Saavedra
• 0
Hi Recently, I used https://www.gensas.org/ to make the annotation. It was pretty practical and in my opinion easy. You just need to up…
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