just now by
BioinfGuru
★
1.7k
0
votes
0
replies
4
views
1
vote
1
reply
207
views
47 minutes ago by
Dinmukhamed
•
0
0
votes
0
replies
15
views
49 minutes ago by
Dinmukhamed
•
0
0
votes
0
replies
36
views
1
vote
2
replies
260
views
updated 4 hours ago by
Pierre Lindenbaum
161k
•
written 15 hours ago by
rahu
•
0
0
votes
0
replies
60
views
5 hours ago by
atharvakarkare14
▴
10
7
votes
3
replies
2.6k
views
updated 5 hours ago by
colindaven
6.3k
•
written 15 months ago by
Dr.Animo
▴
130
795
votes
167
replies
141k
views
109 follow
updated 12 weeks ago by
Biostar
2.6k
•
written 7.4 years ago by
Istvan Albert
100k
3
votes
1
reply
146
views
0
votes
2
replies
361
views
0
votes
2
replies
224
views
11
votes
12
replies
719
views
0
votes
0
replies
88
views
updated 15 hours ago by
GenoMax
141k
•
written 16 hours ago by
N.Y.Wiyana-Hewage
•
0
0
votes
2
replies
229
views
0
votes
2
replies
221
views
9 hours ago by
Ashok
•
0
0
votes
2
replies
245
views
6 hours ago by
Ashok
•
0
5
votes
2
replies
334
views
updated 20 hours ago by
shelkmike
★
1.2k
•
written 1 day ago by
kirillkirilenko
▴
40
0
votes
0
replies
96
views
updated 20 hours ago by
Ram
43k
•
written 20 hours ago by
samuelkalandarov2002
•
0
1
vote
1
reply
254
views
0
votes
1
reply
174
views
updated 21 hours ago by
Ram
43k
•
written 21 hours ago by
_quantum_girl_
▴
10
0
votes
0
replies
103
views
1
vote
4
replies
589
views
0
votes
0
replies
94
views
21 hours ago by
enferdeflame
•
0
0
votes
0
replies
101
views
0
votes
0
replies
106
views
updated 22 hours ago by
Ram
43k
•
written 23 hours ago by
carlopecoraro2
★
2.5k
1
vote
9
replies
638
views
updated 23 hours ago by
Elisabeth Gasteiger
★
2.4k
•
written 1 day ago by
Jacky
•
0
0
votes
1
reply
189
views
0
votes
1
reply
205
views
0
votes
0
replies
106
views
updated 22 hours ago by
Ram
43k
•
written 1 day ago by
carlopecoraro2
★
2.5k
4
votes
2
replies
266
views
1
vote
0
replies
110
views
Tutorial:
DNA Methylation: Sequencing Techniques
0
votes
1
reply
379
views
3
votes
3
replies
373
views
8 hours ago by
SeoGyun
•
0
3
votes
4
replies
384
views
0
votes
3
replies
344
views
0
votes
0
replies
109
views
updated 22 hours ago by
Ram
43k
•
written 1 day ago by
bioinfo223
▴
10
0
votes
0
replies
124
views
1 day ago by
P.
•
0
0
votes
0
replies
124
views
0
votes
1
reply
258
views
updated 11 hours ago by
cmdcolin
★
3.8k
•
written 1 day ago by
renan.igor
•
0
1
vote
1
reply
213
views
updated 1 day ago by
Ram
43k
•
written 1 day ago by
anasjamshed
▴
120
3
votes
1
reply
572
views
updated 1 day ago by
etiennedanis
▴
20
•
written 19 months ago by
DareDevil
★
4.3k
0
votes
0
replies
129
views
0
votes
1
reply
220
views
5
votes
4
replies
539
views
16 hours ago by
BioinfGuru
★
1.7k
1
vote
1
reply
241
views
0
votes
4
replies
463
views
1
vote
1
reply
196
views
updated 1 day ago by
Ram
43k
•
written 1 day ago by
jammydodger123456
▴
40
0
votes
1
reply
314
views
0
votes
2
replies
312
views
1 day ago by
22211020193
•
0
Recent Votes
Recent Locations •
All
UK, just now
Dublin, Ireland, 5 minutes ago
India, 6 minutes ago
Palau, 7 minutes ago
India, 9 minutes ago
France, 10 minutes ago
Taiwan, 12 minutes ago
Recent Awards •
All
Popular Question to
i.sudbery
19k
Popular Question to
biwdpang
•
0
Popular Question to
Michael
54k
Scholar to
Gordon Smyth
★
7.0k
Popular Question to
yueli7
▴
250
Popular Question to
Chirag Parsania
★
2.0k
Recent Replies
Comment: Ciriquant not configuring hisat2 indexed files
by
Atul K.
•
0
it worked. Thank you. Now my terminal is crashing, seems 16gb of RAM and 4 cores isn't enough. Can you suggest something?
Comment: Adding CB tag to bam file
by
Maria
•
0
I checked Picard ```AddOrReplaceReadsGroups``` and ```AddOATag```. Is that what you're referring to?
Comment: Free/open source 23andme-like analysis
by
Michael
54k
Noted that 'accurate' wasn't among your criteria ;)
Anyway, most variant callers are free open-source software. However, for ancestry ana…
Answer: Mapping cDNA sequence of a single gene to a genome not hosted at UCSC server (wi
by
Pierre Lindenbaum
161k
> server so I cannot use Blat for this purpose.
blat is avilable as a standane tool: http://hgdownload.soe.ucsc.edu/admin/exe/linux.x86…
Answer: Mapping cDNA sequence of a single gene to a genome not hosted at UCSC server (wi
by
b.contreras.moreira
▴
160
My favourite for this task would be [GMAP]( http://research-pub.gene.com/gmap ), which can produce GFF format with option `-f 2`.
It is…
Answer: Prediction of Ribosomal RNA Genes Using RNAmmer Software
by
colindaven
6.3k
Another alternative is the truly free implementation barrnap https://github.com/tseemann/barrnap. This is also installable via conda so qui…
Comment: Filtration Of Reads With Length Lower Than 30 From Bam
by
Hagen
▴
10
thanks! This saved me quite some time, I was about to write code to calculate the aligned length from the CIGAR. But this can be done with
…
Comment: Per base sequence content failed miserably
by
Kai Xin
•
0
will look into that. Thank you very much
Comment: Korean human genome reference file
by
SeoGyun
•
0
Thanks..... But I want to do imputation, so I have to make it as a vcf file, but the site only has a fatsa file....
Comment: miRNAs quantification using mirdeep2 tool
by
Ashok
•
0
thank you for your reply
https://github.com/rajewsky-lab/mirdeep2/blob/master/TUTORIAL.md
i saw this github tutorial for miRNA quantifica…
Comment: Load a full GFF3 into annotation track using arrow (Apollo)
by
cmdcolin
★
3.8k
Hi there, I let a developer know of this thread and they said they can check it out next week. Feel free to post to the https://github.com/…
Comment: Removing duplicates
by
joe
▴
510
I'd try those OP @samuel recommends
My guess is you're new to ONT, fast5 is the 'raw data' which is current over time...other than generat…
Comment: RNA seq differential expression analysis
by
swbarnes2
14k
No. Find a tutorial, try it, then if you have specific questions, ask of the galaxy help site.
Traffic: 2483 users visited in the last hour
Use of this site constitutes acceptance of our User Agreement and Privacy Policy.
Powered by the
version 2.3.6
version 2.3.6