Latest
Open
Jobs
Tutorials
Tags
About
FAQ
Community
Planet
New Post
Log In
New Post
Latest
Open
News
Jobs
Tutorials
Forum
Tags
Planet
Users
Log In
Sign Up
About
Limit : all time
all time
today
this week
this month
this year
116,019 results • Page
1 of 2321
Sort: Rank
Rank
Views
Votes
Replies
0
votes
0
replies
6
views
Looking for RPIP Illumina kit probe sequences
Sequences
Illumina
24 minutes ago by
David Tachiquín
• 0
0
votes
2
replies
16
views
Low number of both surviving reads after trimming
trimmomatic
trimming
updated just now by
GenoMax
140k • written 49 minutes ago by
Jay
• 0
2
votes
1
reply
171
views
Forum:
F*up Night style events for Bioinformatics ? Comment if you're interested!
mistakes
updated 57 minutes ago by
Pierre Lindenbaum
160k • written 1 hour ago by
Ann
★ 2.4k
793
votes
167
replies
139k
views
109 follow
News:
The Biostar Handbook. A bioinformatics e-book for beginners.
training
handbook
updated 9 weeks ago by
Biostar
2.6k • written 7.3 years ago by
Istvan Albert
100k
0
votes
2
replies
78
views
ONT direct RNA sequencing
gz
DRS
ONT
gunzip
gzip
updated 1 hour ago by
joe
▴ 470 • written 5 hours ago by
Karina
• 0
0
votes
0
replies
12
views
Number of CPUs to use by DRAP for transcriptomic assmbly
DRAP
CPU
transcriptomics
1 hour ago by
Mohamed Abderrahmane
▴ 10
2
votes
2
replies
29
views
Gene reads all zero for each sample
R
salmon
DE
DESeq2
1 hour ago by
DYLAN NICO
• 0
0
votes
2
replies
142
views
Can I have multiple active GDC download tokens?
TCGA
updated 1 hour ago by
Zhenyu Zhang
★ 1.1k • written 3 days ago by
mrk
• 0
0
votes
0
replies
25
views
Contradictions in GSEA results
GSEA
Enrichment-analysis
PEA
updated 2 hours ago by
Ram
43k • written 2 hours ago by
Tolga
▴ 20
0
votes
0
replies
22
views
Illumina Dna Prep kit for sequencing phage linear genomes
tagmentation
transposons
phagetermini
sequencing
2 hours ago by
alenew.am
• 0
0
votes
3
replies
215
views
FindAllMarkers not working (Error (data layers are not joined. Please run JoinLayersWarning: When testing 1 versus all)
FindAllMarkers
seurat
re-clustering
2 hours ago by
Nitin
• 0
0
votes
1
reply
48
views
(sc)RNA-seq dataset for these cell lines: HEK293, HeLa, PC3 and U2OS
scRNA-seq
updated 2 hours ago by
Ram
43k • written 3 hours ago by
chiditatawmbi
• 0
0
votes
1
reply
60
views
Normalization strategies for comparing mapped reads across samples in RNA-seq
normalization
rnaseq
updated 2 hours ago by
Ram
43k • written 3 hours ago by
Picasa
▴ 640
0
votes
0
replies
44
views
How to change alternate conformation occupancy ?
Bio.PDB.Atom
Bio.PDB
biopython
updated 1 hour ago by
Ram
43k • written 4 hours ago by
udakshi
• 0
0
votes
0
replies
58
views
Training
chip-seq
ScATAC-seq
4 hours ago by
Manhezz
• 0
1
vote
2
replies
86
views
Issue with making local BLAST database
database
mitochodrial
diatoms
blast
ncbi
3 hours ago by
Begonia_pavonina
▴ 140
1
vote
1
reply
264
views
diffbind : killed dba.count(DBsample, bParallel=FALSE)
diffbind
r
atacseq
5 hours ago by
Pierre Lindenbaum
160k
1
vote
2
replies
73
views
Flow Cytometry Data Analysis by Seurat
Seurat
updated 1 hour ago by
Ram
43k • written 5 hours ago by
gdfsnkfns
• 0
1
vote
11
replies
204
views
struggle to get fasta files from ucsc goldenPath
ucsc
getfasta
fasta
updated just now by
GenoMax
140k • written 5 hours ago by
Lila M
★ 1.2k
2
votes
2
replies
73
views
Volcano Plot Output Inquiry: Graphs Facing Down
rstudio
volcano-plot
updated 1 hour ago by
dariober
14k • written 6 hours ago by
Pumla
• 0
0
votes
1
reply
70
views
Merging featureCounts output for different species
featureCount
RNAseq
updated 7 hours ago by
andrebolerbarros
• 0 • written 7 hours ago by
Christian
• 0
2
votes
2
replies
101
views
Interproscan taking so much time
slurm
interproscan
transcriptomics
updated 7 hours ago by
dthorbur
★ 1.7k • written 7 hours ago by
Mohamed Abderrahmane
▴ 10
0
votes
0
replies
48
views
Combine rename and rmdup in SeqKit to remove duplicate sequences and append N in header? Sort by occurence?
seqkit
fasta
7 hours ago by
Broccoli
• 0
0
votes
0
replies
48
views
Is there a good tool/reference for PBP and porin analysis
PBP
porin
7 hours ago by
blur
▴ 280
0
votes
0
replies
55
views
meta-analysis on RNA seq AND microarray results
microarray
RNA
8 hours ago by
liuyibin2005
• 0
49
votes
30
replies
11k
views
13 follow
Tutorial:
How to Use Biostars, Part-I: Questions, Answers, Comments and Replies
documentation
meta
how-to
updated 4 hours ago by
Istvan Albert
100k • written 8.1 years ago by
Ram
43k
0
votes
0
replies
45
views
SPOTlight deconvolution logFC, logFC cohen or mean.AUC
spatial-transcriptomics
SPOTlight
logFC
deconvolution
updated 2 hours ago by
Ram
43k • written 8 hours ago by
M'zelle
• 0
0
votes
5
replies
200
views
calculate mismatch rate from VCF file
mismatch-rate
VCF
updated 2 hours ago by
Ram
43k • written 14 hours ago by
Dora
▴ 10
2
votes
3
replies
265
views
Hybrid assembly Pacbio- Nanopore
long-reads
hybric
assembly
genome
short-reads
updated 9 hours ago by
colindaven
6.3k • written 2 days ago by
Lissa Cruz Saavedra
• 0
0
votes
0
replies
63
views
In scRNA, How should I set QC standards?
scRNA
QC
11 hours ago by
mnx0723
• 0
0
votes
0
replies
54
views
Annotation of Complex genomes using NCBI Genome Workbench
Genome-Workbench
MAKER
updated 2 hours ago by
Ram
43k • written 13 hours ago by
SAsciences
• 0
0
votes
2
replies
226
views
Error occurs when migrate-n software performs "make mpis"
migrate-n
13 hours ago by
lingxue
• 0
0
votes
0
replies
64
views
How to check if a few pathways have relationship?
MSigdb
REACTOME
KEGG
14 hours ago by
Chris
▴ 260
2
votes
10
replies
699
views
Only one read per run - Trying to use CellRangerv7
Fastq
CellRanger
updated 2 hours ago by
Ram
43k • written 5 weeks ago by
Sky
▴ 10
3
votes
2
replies
270
views
FDR and Bonferroni
FDR
Bonferroni
updated 15 hours ago by
joe
▴ 470 • written 2 days ago by
song
• 0
0
votes
1
reply
472
views
Which fasta files are assembled transcripts IDBA_tran assembler ?
RNA-Seq
Assembly
updated 17 hours ago by
weidonglu
• 0 • written 3.8 years ago by
sunnykevin97
▴ 980
0
votes
0
replies
78
views
doing some ontology enrichment analysis
GO
17 hours ago by
aleksk779
• 0
2
votes
1
reply
171
views
Choosing an FC Threshold
statistics
rna-seq
updated 17 hours ago by
Gordon Smyth
★ 6.9k • written 1 day ago by
Netanel
• 0
0
votes
1
reply
110
views
Converting AutoDock-Vina PDBQTs to PDB files
Docking
AutoDock
Vina
PDBQT
PDB
updated 20 hours ago by
Mensur Dlakic
★ 26k • written 1 day ago by
Ricard
• 0
0
votes
2
replies
149
views
novel and know mir156 and mir172
a
updated 17 hours ago by
gayachit
▴ 200 • written 20 hours ago by
Fadmo
• 0
0
votes
1
reply
97
views
Update R in Posit Workbench
R
Posit
updated 21 hours ago by
Ram
43k • written 21 hours ago by
Nitin
• 0
2
votes
8
replies
242
views
Piping samtools to R
R
NGS
bash
samtools
22 hours ago by
joe
▴ 470
8
votes
6
replies
387
views
Pangenome of plant
pangenome
plant
updated 23 hours ago by
talbots
• 0 • written 2 days ago by
analyst
▴ 10
1
vote
2
replies
176
views
vg call is time consuming
vg
23 hours ago by
Maxine
▴ 40
4
votes
14
replies
4.5k
views
8 follow
Installation: Tax4Fun2 package are not found and github repository is not maintained anymore
Tax4Fun2
updated 23 hours ago by
Ram
43k • written 21 months ago by
Elsa
▴ 20
0
votes
8
replies
410
views
STAR aligner options
STAR
7 hours ago by
theophile
• 0
2
votes
3
replies
508
views
Website or tool to obtain a list of genes that participate in a specific process?
genomics
updated 1 day ago by
NancyTLi
• 0 • written 3 months ago by
Hamtaro
▴ 50
0
votes
2
replies
293
views
How to obtain Node/ Edge information from a Reactome Pathway
R
API
pathways
reactome
databases
updated 1 day ago by
GenoMax
140k • written 4 weeks ago by
K.patel5
▴ 140
0
votes
2
replies
158
views
Why counting by diffbind and featurecounts differ?
featurecounts
diffbind
19 hours ago by
Ankit
▴ 500
0
votes
1
reply
104
views
Wanting to make a Venn diagram of ATACseq peak files, unsure of where to start
ATAC-seq
updated 1 day ago by
Yogi
▴ 70 • written 1 day ago by
Ronin
• 0
116,019 results • Page
1 of 2321
Recent Votes
Which human reference genome should I use?
Comment: Centromere and telomere positions for Chm13v2 assembly
C: Last column in UCSC cytoBands.txt files
Answer: F*up Night style events for Bioinformatics ? Comment if you're interested!
F*up Night style events for Bioinformatics ? Comment if you're interested!
Comment: Volcano Plot Output Inquiry: Graphs Facing Down
Comment: Gene reads all zero for each sample
Recent Locations •
All
France,
just now
United States,
1 minute ago
United Kingdom,
3 minutes ago
United States,
3 minutes ago
Bologna,
4 minutes ago
United Kingdom,
6 minutes ago
WCIP | Glasgow | UK,
6 minutes ago
Recent Awards •
All
Popular Question
to
Ann
★ 2.4k
Commentator
to
Devon Ryan
104k
Popular Question
to
amitpande74
▴ 20
Scholar
to
Pierre Lindenbaum
160k
Popular Question
to
beacamara
• 0
Popular Question
to
blur
▴ 280
Popular Question
to
ATpoint
81k
Recent Replies
Comment: struggle to get fasta files from ucsc goldenPath
by
GenoMax
140k
Looks like those appear to be "polycomb associated non-coding RNA". You probably know what they are. https://www.ncbi.nlm.nih.gov/nuccore/…
Comment: Parsing fasta file by coordinates
by
GenoMax
140k
> sequences that are from the same region from those that aren't. could refer to sequence composition and that is the reason we were sugge…
Comment: Low number of both surviving reads after trimming
by
Ram
43k
Please edit your post and add the code parts again, this time use the `101010` code button and NOT the double quote button. The latter is u…
Answer: F*up Night style events for Bioinformatics ? Comment if you're interested!
by
Pierre Lindenbaum
160k
... reminds me [https://twitter.com/yokofakun/status/1267877851795259397][1] ( my twitter account is now private) ![ops][2] [1]: https…
Comment: Flow Cytometry Data Analysis by Seurat
by
Ram
43k
Why did you delete this post, https://www.biostars.org/u/142036/ ?
Comment: Volcano Plot Output Inquiry: Graphs Facing Down
by
dariober
14k
Perhaps more concerning is why you have features (genes?) with small pvalue but fold-change very close to zero. It's also unusual that ther…
Comment: Gene reads all zero for each sample
by
DYLAN NICO
• 0
Thank you for the suggestions. Unfortunately I cannot add any details for lack of information. But I will try using a different alignment a…
Comment: struggle to get fasta files from ucsc goldenPath
by
Lila M
★ 1.2k
They are +ve and -ve strands, but identical two by two ... I can filter that out later on. This is probably a very naive question, but what…
Comment: Gene reads all zero for each sample
by
ATpoint
81k
Do I understand correctly that you have one gene you are interested in, but all counts are zero, right? If so, first thing could be to use …
Answer: ONT direct RNA sequencing
by
joe
▴ 470
You should use `cat` on .gz files, part of the magic of .gz ...I guess if you use `zcat` they are unzipped and this is why you get the erro…
Comment: Nanopore data filtering using fastp
by
emilydolivo97
• 0
thank you !!
Answer: Can I have multiple active GDC download tokens?
by
Zhenyu Zhang
★ 1.1k
You can only have one active token at a time, but you can copy the same token as many times as you want and use them in different computers…
Comment: Parsing fasta file by coordinates
by
sorrymouse
▴ 120
Yes, thats correct. A lot of the responses are addressing problems other than the one I have - I don't want to cluster the sequences based …
Comment: struggle to get fasta files from ucsc goldenPath
by
GenoMax
140k
Not sure if I am missing something but you want 4 copies of the same sequence with a different fasta header?
Comment: Normalization strategies for comparing mapped reads across samples in RNA-seq
by
Ram
43k
> TPM seems like a good method, but it may not be suitable here because it requires gene/transcript length. Also because TPM cannot be com…
Traffic: 2908 users visited in the last hour
Content
Search
Users
Tags
Badges
Help
About
FAQ
Access
RSS
API
Stats
Use of this site constitutes acceptance of our
User Agreement and Privacy Policy
.
Powered by the
version 2.3.6