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116,460 results • Page
2 of 2330
Sort: replies
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Views
Votes
Replies
61
votes
51
replies
22k
views
9 follow
Tool:
Efficiently process (view, analize, clip ends, convert, demultiplex, dereplicate) SFF/FastQ files
sff
sequence
fastq
next-gen
updated 9 months ago by
Ram
43k • written 10.0 years ago by
BioApps
▴ 790
27
votes
50
replies
18k
views
7 follow
Extract fasta sequences from a large file using a list of names
fasta
updated 14 months ago by
Ram
43k • written 9.0 years ago by
fhsantanna
▴ 610
26
votes
50
replies
14k
views
8 follow
Tool:
Retrieve a subset of FASTA from large Illumina multi-FASTA file
fasta
multi-fasta
illumina
updated 9 months ago by
Ram
43k • written 7.9 years ago by
hcwang
▴ 50
188
votes
50
replies
73k
views
41 follow
How To Remove The Same Sequences In The Fasta Files?
fasta
sequence
duplicates
updated 18 months ago by
Ram
43k • written 13.5 years ago by
Zhangleisdau
▴ 340
93
votes
50
replies
16k
views
17 follow
Forum:
Fda Sends A Warning Letter To 23Andme - Personal Genomics Service Marketing To Be Discontinued
personal-genomics
23andMe
updated 13 months ago by
Ram
43k • written 10.4 years ago by
Istvan Albert
100k
12
votes
50
replies
4.1k
views
How can I find out the Linux version and what is the ftp command to install R and R Studio on Linux server?
R
7.1 years ago by
thomas.f.hahn2
▴ 100
92
votes
50
replies
53k
views
16 follow
PCA plot from read count matrix from RNA-Seq
RNA-Seq
pca
updated 2.4 years ago by
BioNovice247
▴ 20 • written 6.4 years ago by
rachel.kubik12
▴ 220
22
votes
50
replies
17k
views
Why I can not install anything on R?
R
software error
5.5 years ago by
Za
▴ 140
85
votes
49
replies
10k
views
31 follow
Which Application Is Truly Missing In Bioinformatics?
subjective
general
updated 5 months ago by
Ram
43k • written 14.1 years ago by
Jarretinha
3.4k
262
votes
48
replies
23k
views
37 follow
Forum:
Bioinformatics courses, workshops or training
Workshop
Training
Courses
updated 13 months ago by
Lars
★ 1.0k • written 8.9 years ago by
Deepak Tanwar
★ 4.2k
72
votes
48
replies
18k
views
15 follow
Tutorial:
For Short Reads, Which Aligners Find All Hits Given Certain Edit Distance Threshold?
ngs
alignment
bwa
updated 13 months ago by
Ram
43k • written 11.2 years ago by
lh3
33k
72
votes
48
replies
8.4k
views
18 follow
What Kind Of Bioinformatics Tutorials Would You Like To See Online?
education
updated 14 months ago by
Ram
43k • written 13.0 years ago by
User 59
13k
68
votes
48
replies
8.7k
views
16 follow
Forum:
Stranger Things: unexpected limitations of popular tools
samtools
hisat2
updated 11 months ago by
Ram
43k • written 6.8 years ago by
Istvan Albert
100k
90
votes
48
replies
33k
views
29 follow
Tutorial:
Extract Total Non-Overlapping Exon Length Per Gene With Bioconductor
fpkm
rna-seq
bioconductor
updated 5 months ago by
Ram
43k • written 10.5 years ago by
Irsan
★ 7.8k
131
votes
48
replies
16k
views
19 follow
Justifying Learning Linux For Bioinformatics
linux
subjective
updated 12.7 years ago by
Guangchuang Yu
★ 2.6k • written 12.7 years ago by
Eric Normandeau
11k
39
votes
48
replies
7.0k
views
CUTADAPT in windows
sequencing
updated 14 months ago by
Ram
43k • written 9.0 years ago by
zizigolu
★ 4.3k
23
votes
47
replies
7.0k
views
6 follow
Dbsnp Individual Genotyping Information For Specific Snps
dbsnp
updated 13.1 years ago by
Khader Shameer
18k • written 13.1 years ago by
Andrea_Bio
★ 2.8k
188
votes
47
replies
2.7k
views
16 follow
Job:
Bioinformatics freelancers needed
freelancer
updated 12 months ago by
Ram
43k • written 8.0 years ago by
Bioinfexperts
▴ 190
22
votes
47
replies
5.2k
views
(solved) I couldn't reproduce the problem of max_target_seqs
blast
updated 5.1 years ago by
gb
★ 2.2k • written 5.5 years ago by
fishgolden
▴ 510
18
votes
47
replies
4.6k
views
Fastq Trimmer by pattern
fastq
trimming
reads
terminal
7.2 years ago by
dzisis1986
▴ 70
176
votes
46
replies
19k
views
26 follow
Forum:
Crac: Funny And/Or Weird Names For Bioinformatics Tools
humor
5 months ago by
Istvan Albert
100k
43
votes
46
replies
4.2k
views
taking sequences of a list of miRNAs
miRNA
mirbase
sequence
gene
updated 7.5 years ago by
Ram
43k • written 7.5 years ago by
zizigolu
★ 4.3k
92
votes
46
replies
12k
views
28 follow
Best Language For Introductory Programming Course From Within An Introduction Course On Bioinformatics.
programming
subjective
updated 14 months ago by
Ram
43k • written 12.8 years ago by
Andra Waagmeester
3.2k
82
votes
46
replies
8.6k
views
15 follow
Tool:
ChatGPT optimized for bioinformatics questions
gpt
updated 5 weeks ago by
LauferVA
4.2k • written 10 months ago by
Sasha
▴ 830
105
votes
46
replies
13k
views
13 follow
Forum:
Why Does Biostar Cover Questions On Epigenetics, But Not Intelligent Design?
epigenetics
updated 13 months ago by
Ram
43k • written 10.6 years ago by
ugly.betty77
★ 1.1k
37
votes
46
replies
6.8k
views
12 follow
Quick Programming Challenge: How Do I Calculate Reference Coverage From A Table Of Alignment Starts And Ends?
code
alignment
programming
updated 5.6 years ago by
Ram
43k • written 14.0 years ago by
Jeremy Leipzig
22k
125
votes
46
replies
50k
views
21 follow
Forum:
Visualization of ChIP-seq data using Heatmaps (Updated: 06/10/16)
visualization
ChIP-Seq
homer
ngsplot
deeptools
updated 12 months ago by
Ram
43k • written 8.1 years ago by
Sinji
★ 3.2k
237
votes
45
replies
72k
views
34 follow
What Is The Best Pipeline For Human Whole Exome Sequencing?
next-gen
sequencing
illumina
exome
human
updated 12.8 years ago by
User 9126
▴ 50 • written 13.9 years ago by
Biomed
5.0k
22
votes
45
replies
13k
views
9 follow
Tool to separate human and mouse rna seq reads
RNA-Seq
next-gen-sequencing
updated 15 months ago by
Ram
43k • written 8.9 years ago by
Ron
★ 1.2k
108
votes
45
replies
86k
views
30 follow
How To Extract A Sequence From A Big (6Gb) Multifasta File ?
fasta
updated 19 months ago by
aswinssoman
▴ 70 • written 11.7 years ago by
Mchimich
▴ 320
185
votes
45
replies
32k
views
27 follow
How To Organize A Pipeline Of Small Scripts Together?
pipeline
general
updated 6 months ago by
Ram
43k • written 14.1 years ago by
Giovanni M Dall'Olio
28k
4
votes
45
replies
7.7k
views
SAM file wrong? help with validatesamfile
EXOME
updated 12 months ago by
Ram
43k • written 7.8 years ago by
cristina_sabiers
▴ 110
122
votes
44
replies
11k
views
24 follow
News:
Help Make Biostar Better! Add Your Feedback, Opinions And Suggestions.
meta
biostars
updated 5 months ago by
Ram
43k • written 11.4 years ago by
Istvan Albert
100k
102
votes
44
replies
9.0k
views
22 follow
What Are Numbers Every Bioinformatician Should Know?
knowledge
career
updated 13 months ago by
Ram
43k • written 10.7 years ago by
brentp
24k
185
votes
44
replies
83k
views
30 follow
Drawing Chromosome Ideograms With Data
ideogram
visualization
chromosome
updated 29 days ago by
Michael
54k • written 14.1 years ago by
Yuri
★ 1.7k
196
votes
44
replies
71k
views
33 follow
Tutorial:
Analysing Microarray Data In Bioconductor
microarray-analysis
bioconductor
updated 15 months ago by
Ram
43k • written 11.6 years ago by
Obi Griffith
20k
99
votes
44
replies
24k
views
33 follow
Best Free Text Editor For All Popular Languages (R, Python, Perl Etc..)
text
r
perl
python
updated 6.6 years ago by
grant.hovhannisyan
★ 2.6k • written 12.1 years ago by
John
★ 1.5k
90
votes
44
replies
60k
views
28 follow
Tool to generate proportional Venn Diagrams?
visualization
updated 24 months ago by
Ram
43k • written 13.0 years ago by
Ryan D
★ 3.4k
43
votes
44
replies
5.6k
views
14 follow
Macbook M1 pro/M2 pro or Dell (with Ubuntu/other linux distribution) for bioinformatics stuff?
metagenomics
amplicon
macbook
Intel
MacOs
updated 6 months ago by
steve
★ 3.5k • written 6 months ago by
l.gallucci
▴ 10
130
votes
44
replies
34k
views
16 follow
Forum:
Trimming adapter sequences - is it necessary?
NGS
adapter
RNA-Seq
updated 13 months ago by
Ram
43k • written 7.6 years ago by
Lars
★ 1.0k
21
votes
44
replies
4.3k
views
How to analyze Illumina RNASeq data
RNA-Seq
sequencing
next-gen
3.7 years ago by
Kumar
▴ 170
121
votes
44
replies
21k
views
21 follow
What Is Your Favorite Question To Ask When Interviewing Potential Bioinformaticians?
subjective
updated 14 months ago by
Ram
43k • written 13.8 years ago by
Madelaine Gogol
5.3k
27
votes
44
replies
14k
views
How to remove contamination from the transcriptome assembly
RNA-Seq
contamination
assembly
blast
Hiseq reads
updated 20 months ago by
Ram
43k • written 8.4 years ago by
seta
★ 1.9k
155
votes
44
replies
32k
views
29 follow
Which Bioinformatic Friendly Pipeline Building Framework?
scripting
updated 21 months ago by
Ram
43k • written 10.2 years ago by
Carlos Borroto
★ 2.1k
46
votes
44
replies
24k
views
9 follow
bash loop for alignment RNA-seq data
RNA-Seq
next-gen
alignment
updated 19 months ago by
Ram
43k • written 10.0 years ago by
Paul
★ 1.5k
1
vote
43
replies
3.9k
views
Database from .gbk files
database
gbk
ncbi
updated 12 months ago by
Raul
• 0 • written 3.8 years ago by
graysonford
▴ 10
66
votes
43
replies
27k
views
23 follow
Ngs - Huge (Fastq) File Parsing - Which Language For Good Efficiency ?
next-gen
sequencing
parsing
fastq
programming
updated 2.2 years ago by
Ram
43k • written 13.2 years ago by
toni
★ 2.2k
31
votes
43
replies
19k
views
11 follow
Volcano plot help code
R
rna-seq
updated 5.8 years ago by
jordi.planells
▴ 480 • written 6.0 years ago by
anasofiamoreira94
▴ 80
16
votes
43
replies
6.7k
views
Stuck on calculating principal components
Vegan
R
software error
SNP
updated 6.4 years ago by
pfs
▴ 280 • written 6.4 years ago by
zizigolu
★ 4.3k
19
votes
43
replies
5.5k
views
Annotation of huge number of CNV files
CNV annotation TCGA
5.7 years ago by
nazaninhoseinkhan
▴ 520
116,460 results • Page
2 of 2330
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What Is A "Lane" In Next Generation Sequencing Context?
Can I liftover hs37d5 to hg38 using hg19ToHg38 chain file?
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Recent Replies
Answer: Assume "yes" as the answer to any prompts with bioconda
by
Juke34
8.5k
From my experience using `biocontainers` base container no need to set `yes` installation is done without any command prompt, otherwise as …
Comment: SLURM submission problem: Indexing the Reference Genome is not done in STAR
by
Dave Carlson
★ 1.7k
You're allocating 4 compute nodes (`#SBATCH -N 4`), but STAR can't actually make use of more than 1 compute node. You can increase the num…
Answer: Assume "yes" as the answer to any prompts with bioconda
by
Dave Carlson
★ 1.7k
```conda install -y bioconda::packageX conda-forge::packageY``` should do what you're looking for.
Comment: Supergnova stuck in tutorial
by
Murat
• 0
Hi GenoMax, Thank you for your advice. I sent an email to them and have already opened an issue on Github. Let see if there is any other so…
Comment: How can I retrieve wheat lysine non-acetylated sequences from the UniProt databa
by
me
▴ 750
You can't. The data does not exist. UniProt only annotates where a lysine is know to be acetylated. There is no way that UniProt can know w…
Comment: How can I retrieve wheat lysine non-acetylated sequences from the UniProt databa
by
Jacky
• 0
Yes, that's what I meant.
Comment: How can I retrieve wheat lysine non-acetylated sequences from the UniProt databa
by
Jacky
• 0
I'm sorry, I didn't explain it clearly. Below are my operational steps: I enter 'Triticum aestivum' in the search box and perform a search.…
Comment: Indexing the Reference Genome is not done in STAR
by
n_navy
• 0
I add these parameters; #SBATCH -N 4 #SBATCH --tasks=1 #SBATCH -c 4 #SBATCH --time=24:00:00 But it keeps giving error
Comment: RNA seq differential expression analysis
by
rrehimi
• 0
Thanks! Could you please explain the DEseq2 method using Galaxy step by step? Input files?
Answer: Korean reference file
by
colindaven
6.3k
Is is this one - KOREF ? There might be multiple versions. https://www.ncbi.nlm.nih.gov/bioproject/PRJNA294231/ If it is, then take that…
Comment: How can I retrieve wheat lysine non-acetylated sequences from the UniProt databa
by
me
▴ 750
The question is not quite clear? are you asking for wheat proteins in UniProtKB which contain a lysine amino-acid, where those lysine are *…
Comment: RNA seq differential expression analysis
by
seta
★ 1.9k
You should provide row count (without normalization) for doing DEG analysis by relevant tools such as, edgeR or DEseq.
Comment: GSEA analysis in R
by
Sudip
• 0
Thank you. I have another question. Is there a way to get the gene list under "setSize" from the output that you gave as an example.
Comment: Per base sequence content failed miserably
by
Kai Xin
• 0
Hi, i have added the fastqc plots (untrimmed and trimmed). The sample was from anaerobes enriched from wastewater treatment plant, and this…
Comment: How can I retrieve wheat lysine non-acetylated sequences from the UniProt databa
by
Pierre Lindenbaum
161k
> lysine non-acetylated sequences what does it look like in uniprot ? give us an example with a uniprot identifier.
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