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246 results • Page
1 of 5
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Votes
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76
votes
72
replies
19k
views
19 follow
Tutorial:
Clustering of DAVID gene enrichment results from gene expression studies
enrichment
david
3 minutes ago by
Kevin Blighe
87k
54
votes
12
replies
28k
views
12 follow
Out Of Disk Space With Picard Tools ?
picard
updated 6 days ago by
kjngo
• 0 • written 12.0 years ago by
Gabriel R.
★ 2.9k
34
votes
16
replies
20k
views
9 follow
Filtration Of Reads With Length Lower Than 30 From Bam
read
length
bam
updated 5 days ago by
Hagen
▴ 10 • written 10.2 years ago by
filipzembol
▴ 180
25
votes
11
replies
3.7k
views
6 follow
Forum:
Site Use Guidelines
meta
biostars
Guidelines
updated 1 day ago by
Ram
43k • written 14.6 years ago by
Istvan Albert
100k
22
votes
18
replies
6.1k
views
about batch correction in scRNA-seq
scRNA
scRNA-seq
batch-effect
updated 6 days ago by
Ram
43k • written 4.6 years ago by
Bogdan
★ 1.4k
14
votes
2
replies
7.0k
views
Trouble with Pilon installation
polishing
pilon
installation
updated 3 hours ago by
Lissa Cruz Saavedra
• 0 • written 7.3 years ago by
Rox
★ 1.4k
11
votes
12
replies
933
views
Downloaded pdb's on rcsb.org
rcsb
pdb
updated 6 days ago by
Ram
43k • written 6 days ago by
iamsmor
• 0
10
votes
6
replies
14k
views
7 follow
How To Identify The 16S Rrna Gene Sequence In A Genome
updated 6 days ago by
colindaven
6.4k • written 10.9 years ago by
Ontheway
▴ 10
9
votes
3
replies
6.9k
views
EdgeR-GLMs correcting for batch effects
RNA-Seq
edgeR
Batch-Effect
updated 6 days ago by
Ram
43k • written 9.9 years ago by
Matina
▴ 250
9
votes
10
replies
6.9k
views
Harmony batch correction vs. regressing out donor effect in Seurat
Seurat
scRNA-seq
Harmony
RNA-seq
batch-effect
updated 6 days ago by
Ram
43k • written 4.5 years ago by
Lucy
▴ 140
8
votes
5
replies
4.9k
views
ISCN annotation for SV/CN VCF files
SV
written 7 months ago by
a.beggs
▴ 60
7
votes
6
replies
447
views
Filtering sam or bam file with maximum matching region
crisper-edited
alignment
match
3 days ago by
analyst
▴ 30
7
votes
3
replies
2.8k
views
Tutorial:
Prediction of Ribosomal RNA Genes Using RNAmmer Software
Annotation
rRNA
RNAmmer
Genome
updated 5 days ago by
colindaven
6.4k • written 15 months ago by
Dr.Animo
▴ 130
7
votes
2
replies
221
views
Gene set enrichment analysis differences between 2020 and 2024
Gene-ontology
updated 1 day ago by
geneontologyhelp
▴ 390 • written 2 days ago by
catherine.teyssier
• 0
6
votes
7
replies
1.6k
views
How to assess RNA-Seq batch correction success?
Combat
RNA-Seq
Limma
DESeq2
batch-effect
updated 6 days ago by
Ram
43k • written 4.9 years ago by
borkk
▴ 140
6
votes
0
replies
2.6k
views
Tool:
Immuno-Navigator, a batch-corrected database for gene coexpression in the immune system
gene-co-expression
batch-effect
immunology
updated 6 days ago by
Ram
43k • written 7.0 years ago by
alexis.vandenbon
▴ 80
6
votes
3
replies
161
views
Interpretting IGV output
IGV
updated 2 days ago by
Carlo Yague
8.7k • written 2 days ago by
analyst
▴ 30
5
votes
4
replies
616
views
DEG analysis of RNA-seq data across multiple tissues and two conditions
RNA-seq
EdgeR
DEGs
DESeq2
6 days ago by
BioinfGuru
★ 1.7k
5
votes
4
replies
612
views
How to compare the quality of assemblies
nextdenovo
assembly
hifiasm
pacbio
20 hours ago by
kirillkirilenko
▴ 40
5
votes
2
replies
2.6k
views
How do you evaluate the effectiveness of the batch effect algorithm?
Batch-Effect
updated 6 days ago by
Ram
43k • written 9.1 years ago by
chengzhao41
▴ 110
5
votes
3
replies
701
views
Illumina reads preprocessing best practice for snp calling applications
Illumina
snp
updated 5 days ago by
Enrique
• 0 • written 9 months ago by
Denis
▴ 310
5
votes
13
replies
1.1k
views
Tutorial:
Installing/switching between versions of R/Rstudio/Bioconductor on personal machine (Linux | Ubuntu)
R
Ubuntu
Linux
Bioconductor
Rstudio
updated 4 days ago by
ATpoint
82k • written 5 days ago by
BioinfGuru
★ 1.7k
4
votes
5
replies
265
views
To get p-values for the TPM
P-value
TPM
RSEM
updated 8 hours ago by
swbarnes2
14k • written 20 hours ago by
VITALA
• 0
4
votes
2
replies
3.8k
views
DESeq2 design and batch effect
RNA-Seq
batch-effect
DESeq2
Salmon
updated 6 days ago by
Ram
43k • written 6.5 years ago by
georgewwp
▴ 30
4
votes
11
replies
467
views
Redirection of Duplicate PMIDs
pubmed
pmid
updated 1 day ago by
LauferVA
4.2k • written 2 days ago by
dominickd
• 0
4
votes
9
replies
2.6k
views
Tool:
CANEapp, a user-friendly application for automated next generation transcriptomic data analysis
RNA-Seq
written 8.2 years ago by
dvelmeshev
• 0
4
votes
2
replies
311
views
Assume "yes" as the answer to any prompts with bioconda
docker
container
bioconda
updated 6 days ago by
Juke34
8.5k • written 6 days ago by
njornet
▴ 20
3
votes
9
replies
784
views
How can I retrieve wheat lysine non-acetylated sequences from the UniProt database?
UniProt
updated 6 days ago by
Elisabeth Gasteiger
★ 2.4k • written 6 days ago by
Jacky
• 0
3
votes
8
replies
4.9k
views
An idea to identify batch effects
microarray
batch-effect
updated 6 days ago by
Ram
43k • written 9.7 years ago by
mjarosz
• 0
3
votes
6
replies
294
views
PCA plot (Suggestions Needed)
DESeq2
PCAplot
updated 6 hours ago by
swbarnes2
14k • written 21 hours ago by
Aaliya
▴ 10
3
votes
3
replies
3.0k
views
Quick way to Identify batch effect from covariates?
bioconductor
batch-effect
updated 6 days ago by
Ram
43k • written 7.9 years ago by
James Ashmore
★ 3.4k
3
votes
9
replies
386
views
Is it Acceptable to Have Uniform Quality Scores in a FASTQ File?
FASTQ
updated 13 hours ago by
Istvan Albert
100k • written 2 days ago by
Κοσμάς
• 0
3
votes
2
replies
4.1k
views
Batch effects in sequencing data
batch-effect
updated 6 days ago by
Ram
43k • written 8.7 years ago by
Andrew
▴ 60
3
votes
13
replies
623
views
How to convert plink files to Hapmap Format
GWAS
Plink
updated 9 hours ago by
bk11
★ 2.3k • written 7 weeks ago by
Sofia
• 0
3
votes
0
replies
102
views
News:
Interested in Spatial Omics?
Bioconductor
Spatial-Omics
Spatial-Transcriptomics
updated 1 day ago by
Ram
43k • written 1 day ago by
carlopecoraro2
★ 2.5k
3
votes
3
replies
174
views
Genbank vs SRA
ncbi
genbank
genomics
sra
updated 2 days ago by
Mensur Dlakic
★ 27k • written 2 days ago by
c_u
▴ 520
3
votes
3
replies
187
views
Forum:
Ideal PC configurations and operating system for bioinformatics laboratory
PC
updated 1 day ago by
GenoMax
141k • written 1 day ago by
Estevão
• 0
3
votes
8
replies
4.1k
views
Using FPKM and TPM values for batch correction for Single Cell RNA-Seq
fpkm
scRNA-seq
RNA-Seq
tpm
batch-effect
updated 6 days ago by
Ram
43k • written 4.6 years ago by
hkarakurt
▴ 180
3
votes
2
replies
259
views
p-value and Boneferroni adjusted p-value the same in R
Bonferroni
R
Statistics
p-value
Fisher
2 days ago by
kristiewyleung
• 0
3
votes
4
replies
643
views
Mapping cDNA sequence of a single gene to a genome not hosted at UCSC server (without Blat)
mapping
2 days ago by
rahu
• 0
3
votes
4
replies
3.4k
views
any recommended tools for batch effect and normalization not written in R?
R
RNA-Seq
combat
python
batch-effect
updated 6 days ago by
Ram
43k • written 7.1 years ago by
dyollluap
▴ 310
3
votes
3
replies
461
views
Free/open source 23andme-like analysis
SNPs
genomics
NGS
VCF
updated 4 days ago by
swbarnes2
14k • written 5 days ago by
joe
▴ 510
3
votes
4
replies
421
views
biomaRt error for different R version
biomaRt
R
updated 6 days ago by
GenoMax
141k • written 7 days ago by
manaswwm
▴ 490
3
votes
8
replies
1.2k
views
Cellranger count error: Input FASTQ file ended prematurely
scRNA-seq
Cellranger
STARsolo
10X
updated 4 days ago by
swbarnes2
14k • written 9 months ago by
bp22
▴ 80
3
votes
5
replies
237
views
Per base sequence content error in RNAseq analysis
RNA-seq
analysis
updated 2 days ago by
GenoMax
141k • written 2 days ago by
ahmad.sajad4541
• 0
3
votes
5
replies
528
views
SLURM submission problem: Indexing the Reference Genome is not done in STAR
align
RNA-Seq
linux
STAR
2 days ago by
n_navy
• 0
3
votes
3
replies
709
views
KissDE and batch effect
kissDE
kissplice
2 days ago by
david.b.rombaut
▴ 10
3
votes
6
replies
625
views
Ciriquant not configuring hisat2 indexed files
ciriquant
2 days ago by
Atul K.
• 0
3
votes
3
replies
498
views
Korean human genome reference file
GWAS
reference
imputation
5 days ago by
SeoGyun
• 0
3
votes
3
replies
196
views
Getting the nucleotide sequence of proteins, having their UniProt IDs
Nucleotide
Genomic
Sequence
Uniprot
Proteins
updated 2 days ago by
GenoMax
141k • written 2 days ago by
Mariana
▴ 10
246 results • Page
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Recent Votes
Answer: clustalo - align milions of short seq, memory issue
Comment: downloading genomes in fasta format from accession ids
Comment: PCA plot (Suggestions Needed)
Answer: PCA plot (Suggestions Needed)
PCA plot (Suggestions Needed)
Comment: To get p-values for the TPM
A: Where To Download Pam50 Gene Set?
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★ 2.5k
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Answer: Heatmap and rna-seq
by
Kevin Blighe
87k
Hi, you can take the list of GSEA pathways and then use these via GSVA (https://github.com/rcastelo/GSVA) to create a heatmap. You hav…
Comment: Clustering of DAVID gene enrichment results from gene expression studies
by
Kevin Blighe
87k
You will have to elaborate on what is the error
Comment: clustalo - align milions of short seq, memory issue
by
emmanouil.a
▴ 120
Hi GenoMax and Mensur Dlakic, thank you for your feedback. I'm changing approach in the way to work with a smaller number of seq.
Comment: Annovar using R package
by
Pierre Lindenbaum
161k
duplicate of your previous question: https://www.biostars.org/p/9592646/ why using R when there are shell-based tools like VEP ? slivar , …
Comment: Trouble with Pilon installation
by
Lissa Cruz Saavedra
• 0
Hi I am trying to install the software but I didn't find the way to clone from github. Could you help me with the pipeline of instruction…
Comment: Adding CB tag to bam file
by
swbarnes2
14k
I think both these programs are just adding the same tag to every line. I don't think either cares whether it's called RG or CB
Comment: PCA plot (Suggestions Needed)
by
swbarnes2
14k
Even if this was real data, the tiny numbers for the PC1 and PC2 indicate that the differences between the samples are all absolutely tiny.…
Comment: Creating Synthetic Sequences for a ML Model
by
biochugs
• 0
Thanks for the input. I'll continue to think of more ideas that may be achievable.
Comment: PCA plot (Suggestions Needed)
by
Jeremy
▴ 890
You could also try kernel PCA, which is a non-linear form of PCA, although this will be more time-consuming.
Comment: Troubleshooting RNA-seq data with DNA contamination
by
DKA
▴ 40
I have unstranded RNA-seq data. Please share the tool with me, if you do not mind.
Comment: PCA plot (Suggestions Needed)
by
Mensur Dlakic
★ 27k
Everything that @f1f58aed said, plus that there may be no data manipulation that will give you the clusters you expected. Sometimes there a…
Answer: Creating Synthetic Sequences for a ML Model
by
Mensur Dlakic
★ 27k
It is a good idea, though I don't see how it would work in practice. I think you will encounter at least one additional problem on top of …
Answer: PCA plot (Suggestions Needed)
by
Jeremy
▴ 890
Right now, the WT samples are clustering together, but you have one mutant sample off by itself, which could be an outlier. How did you sc…
Comment: Adding CB tag to bam file
by
GenoMax
141k
@yokofakun had written this: https://www.biostars.org/p/336965/ https://github.com/ruqianl/appendCB may also be useful.
Comment: Troubleshooting RNA-seq data with DNA contamination
by
GenoMax
141k
Please post information about your tool as a `tools` category post. Don't wait/ask other users to ask you.
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