I have Whole Genome sequencing DNA data of children sarcoma tumor ( many types of sarcoma i have)
In research paper, i read the children are more affected by heredity factors than environment factors.
Thus, i'm gonna prepare Germline, Somatic calling pipeline, and also copy number.
additionally, based on the children cancer have long term cure need, I have necessity of finding recurrent mutation.
Thus i found the paper
Whole-genome sequencing identifies recurrent mutations in chronic lymphocytic leukaemia
and i'm gonna use tools or pipeline in that paper.
Because i'm the new on bioinformatics, i cannot sure what my plan is logically significant. Thank you.