Entering edit mode
4.9 years ago
sabaghianamir70
▴
70
i want to identify and separate miRNAs from my Raw data. how should i do that? in fact after i analyze the data what tools and options should i use to identify and separate microRNA from my data. for example after QC the Data, should i use Star with some specific options for this matter or should i use some specific tool? help me if anyone know how to do this
I think it would be useful if you mention "what" your data is, how it was generated.
its Rna SEQ single end illumina data generated with nextseq 500. cell line is blood. after Triming i used Star and feature count. but i donw know it is a correct work flow or the options of these tool are correct also or not.