Entering edit mode
5.0 years ago
whlei
•
0
I got alignment by using hisat2 with reference genome, and sort the sam file to bam file. when I used cufflinks to assemble the transcriptome, it gave the error
BAM record error: found spliced alignment without XS attribute
and also it gave four result files,genes.fpkm_tracking, isoforms.fpkm_tracking, skipped.gtf and transcripts.gtf file.My command is as follows:
cufflinks -p 2 --library-type fr-unstranded -o ly1_assemble_out ly1_sort.bam
who can help me how to fix the error? and should I trust the results?
