Tool:Using NGS replicates for variant calling

Question

[swkim]

Hi,

I am introducing our recent software RePlow that detects low-frequent (VAF<1%) somatic mutations using technical replication for NGS sequencing.

https://www.nature.com/articles/s41467-019-09026-y

I think our paper explains why calling mutations with <1% VAF is generally difficult, and what is the best way to resolve the problems. Hope you enjoy this!