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1,163 results • Page
1 of 24
Sort: Votes
Rank
Views
Votes
Replies
362
votes
82
replies
144k
views
35 follow
Tool:
Gnu Parallel - Parallelize Serial Command Line Programs Without Changing Them
parallel
next-gen
ngs
updated 28 days ago by
jena
▴ 290 • written 11.2 years ago by
ole.tange
★ 4.4k
164
votes
70
replies
60k
views
34 follow
How To Efficiently Parse A Huge Fastq File?
fastq
next-gen-sequencing
updated 26 days ago by
Ram
43k • written 12.8 years ago by
Panos
★ 1.8k
103
votes
42
replies
31k
views
34 follow
Forum:
List of cloud genomics companies
cloud-genomics
updated 14 days ago by
Jeremy Leipzig
22k • written 10.4 years ago by
14134125465346445
★ 3.6k
98
votes
106
replies
46k
views
26 follow
Tutorial:
Produce PCA bi-plot for 1000 Genomes Phase III - Version 2
1000genomes
PLINK
PCA
updated 15 days ago by
psb7
▴ 20 • written 5.6 years ago by
Kevin Blighe
87k
87
votes
24
replies
23k
views
17 follow
Tutorial:
Gene Set Enrichment Analysis
microarray
enrichment
RNA-Seq
GSEA
updated 5 days ago by
Gordon
• 0 • written 3.5 years ago by
Hamid Ghaedi
3.2k
81
votes
27
replies
20k
views
12 follow
ATAC-seq sample normalization
ChIP-Seq
atac-seq
normalization
updated 20 days ago by
sgv
• 0 • written 4.3 years ago by
Flo
▴ 240
76
votes
71
replies
19k
views
19 follow
Tutorial:
Clustering of DAVID gene enrichment results from gene expression studies
enrichment
david
updated 1 day ago by
GenoMax
141k • written 6.2 years ago by
Kevin Blighe
87k
57
votes
22
replies
28k
views
7 follow
Batch correction in DESeq2
Combat
DESeq2
Batch-Effect
updated 17 days ago by
Ram
43k • written 4.5 years ago by
Arindam Ghosh
▴ 510
54
votes
12
replies
28k
views
12 follow
Out Of Disk Space With Picard Tools ?
picard
updated 5 days ago by
kjngo
• 0 • written 12.0 years ago by
Gabriel R.
★ 2.9k
49
votes
30
replies
11k
views
13 follow
Tutorial:
How to Use Biostars, Part-I: Questions, Answers, Comments and Replies
documentation
meta
how-to
updated 26 days ago by
Istvan Albert
100k • written 8.1 years ago by
Ram
43k
39
votes
9
replies
42k
views
6 follow
Batch effects : ComBat or removebatcheffects (limma package) ?
limma
sva
Combat
batch-effect
updated 6 days ago by
Ram
43k • written 6.7 years ago by
lessismore
★ 1.3k
37
votes
5
replies
13k
views
How do you generate TMM normalized counts using EdgeR?
R
RNA-seq
edgeR
updated 13 days ago by
inedraylig
▴ 60 • written 2.9 years ago by
Pratik
★ 1.0k
34
votes
16
replies
20k
views
9 follow
Filtration Of Reads With Length Lower Than 30 From Bam
read
length
bam
updated 4 days ago by
Hagen
▴ 10 • written 10.2 years ago by
filipzembol
▴ 180
31
votes
14
replies
12k
views
11 follow
Finding Rrna Genes In Metagenomic Data
rna
rRNA
metagenomics
updated 28 days ago by
Ram
43k • written 13.5 years ago by
toshnam
▴ 650
30
votes
12
replies
3.8k
views
Trying to understand the maths behind one Limma function
limma
RNA-Seq
batch-effect
updated 8 days ago by
Ram
43k • written 4.0 years ago by
Mozart
▴ 330
23
votes
23
replies
9.8k
views
6 follow
How to see if adjusting batch effect in RNA-seq is working or not
RNA-Seq
removeBatchEffect
batch-effect
edger
updated 6 days ago by
Ram
43k • written 5.3 years ago by
iraia.munoa
▴ 130
23
votes
11
replies
4.8k
views
7 follow
Code golf: detecting homopolymers of length N in the (human) genome
code golf
fasta
homopolymer
repeat
updated 7 days ago by
Ram
43k • written 4.9 years ago by
WouterDeCoster
47k
22
votes
14
replies
28k
views
7 follow
removing batch effects using ComBat and SVA
SVA
ComBat
R
Batch-effect
updated 15 days ago by
Ram
43k • written 7.9 years ago by
LJ
▴ 280
22
votes
18
replies
6.1k
views
about batch correction in scRNA-seq
scRNA
scRNA-seq
batch-effect
updated 5 days ago by
Ram
43k • written 4.6 years ago by
Bogdan
★ 1.4k
18
votes
20
replies
4.4k
views
6 follow
How to identify significant differentially expressed genes and gene regulatory networks from microarray data.
microarray
differential-gene-expression
updated 14 days ago by
Ram
43k • written 7.5 years ago by
morteza.mahmoudisaber
▴ 80
17
votes
16
replies
14k
views
10 follow
How to extract the longest isoform from multi fasta file
perl
extract
fasta
python
isoform
updated 20 days ago by
Madeleine
• 0 • written 9.7 years ago by
jon.brate
▴ 310
17
votes
8
replies
12k
views
9 follow
MAF vs VAF
sequencing
updated 20 days ago by
AshleeThomson
▴ 80 • written 6.1 years ago by
lauren.wahyudi
▴ 50
16
votes
10
replies
7.6k
views
6 follow
Intersect multiple BED files
bed
intersect
updated 27 days ago by
Alex Reynolds
35k • written 8.3 years ago by
int11ap1
▴ 470
16
votes
10
replies
1.0k
views
6 follow
Forum:
F*up Night style events for Bioinformatics ? Comment if you're interested!
mistakes
updated 25 days ago by
Madelaine Gogol
5.3k • written 26 days ago by
Ann
★ 2.4k
15
votes
6
replies
1.3k
views
6 follow
VCF to human friendly form?
vcf
visualization
updated 8 days ago by
Pierre Lindenbaum
161k • written 19 months ago by
wormball
▴ 10
13
votes
10
replies
6.4k
views
Adjusting for confounding variables in RNA-SEQ Differential Expression Analysis
Differential-Gene-Expression
RNA-Seq
Statistics
updated 14 days ago by
Ram
43k • written 5.7 years ago by
ucakhnd
▴ 40
13
votes
13
replies
18k
views
9 follow
How Do I Translate Multiple (More Than 25000) Dna Sequences With Different Frames To Protein Seuquence?
dna
protein
updated 15 days ago by
ummeswaiba
• 0 • written 10.3 years ago by
biostar
▴ 170
13
votes
22
replies
11k
views
SVA : Setting up the data from an ExpressionSet and applying sva function to extimate batches
sva
combat
batch-effect
updated 6 days ago by
Ram
43k • written 6.8 years ago by
lessismore
★ 1.3k
12
votes
2
replies
435
views
News:
8th Berlin Summer School in NGS Data Analysis (June 10-14, 2024) - already half booked
Workshop
RNA-Seq
DNA-Seq
Illumina
13 days ago by
David Langenberger
11k
12
votes
14
replies
2.4k
views
8 follow
Forum:
Recovering bam files after unknown deletion in the storage
photorec
bam
sequencing
written 4.9 years ago by
Gabriel Wajnberg
▴ 70
12
votes
13
replies
4.4k
views
RNA-Seq DGE using PCA components as covariate
differential-gene-expression
RNA-Seq
glm
DESeq2
updated 14 days ago by
Ram
43k • written 6.2 years ago by
firestar
★ 1.6k
12
votes
17
replies
6.0k
views
9 follow
ACMG assignment tools
wes
acmg
WES
WGS
updated 19 days ago by
Zuber
• 0 • written 3.9 years ago by
Eugene A
▴ 180
12
votes
4
replies
2.2k
views
long read + salmon? (transcript abundance)
long-read
salmon
updated 18 days ago by
Ram
43k • written 13 months ago by
shinyjj
▴ 50
12
votes
7
replies
7.0k
views
Forum:
Differences between SMART-seq2, SMART-seq3, and 10x
cell
smartseq
UMIs
single
10x
updated 29 days ago by
dsull
★ 5.8k • written 2.6 years ago by
hamarillo
▴ 70
11
votes
12
replies
926
views
Downloaded pdb's on rcsb.org
rcsb
pdb
updated 4 days ago by
Ram
43k • written 5 days ago by
iamsmor
• 0
11
votes
9
replies
6.9k
views
How to choose method to integrate different patient sample together in Seurat
Seurat
scRNA
batch-effect
harmony
updated 6 days ago by
Ram
43k • written 4.2 years ago by
623202215
▴ 80
11
votes
6
replies
507
views
Pangenome of plant
pangenome
plant
15 days ago by
analyst
▴ 20
11
votes
22
replies
4.2k
views
No significant DEG: A request to double check my commands for limma.
limma
differential-gene-expression
updated 14 days ago by
Ram
43k • written 5.0 years ago by
RNAseqer
▴ 260
11
votes
11
replies
1.1k
views
7 follow
How I know if it is a batch effect or are real differences between my controls and treatments?
RNA-seq
Transcriptomics
ComBat-seq
Batch-Effect
updated 14 days ago by
GenoMax
141k • written 17 days ago by
alejandro.mejiam1
▴ 10
11
votes
9
replies
11k
views
10 follow
Genome assembly statistical tools
Assembly
statistics
updated 10 days ago by
skdv2522
• 0 • written 4.7 years ago by
margab
▴ 10
10
votes
6
replies
14k
views
7 follow
How To Identify The 16S Rrna Gene Sequence In A Genome
updated 5 days ago by
colindaven
6.4k • written 10.9 years ago by
Ontheway
▴ 10
10
votes
6
replies
4.1k
views
Tutorial:
CorLevelPlot - Visualise correlation results, e.g., clinical parameter correlations
correlation
corlevelplot
updated 27 days ago by
Ram
43k • written 6.2 years ago by
Kevin Blighe
87k
10
votes
37
replies
12k
views
21 follow
Convert Gene IDs of Tomato Sol Database
tomato
gene
updated 13 days ago by
aziz.hammou
• 0 • written 8.8 years ago by
kevluv93
▴ 170
10
votes
5
replies
1.4k
views
6 follow
Courses in RNAseq data
RNA-seq
Courses
updated 21 days ago by
Ram
43k • written 2.5 years ago by
margot.27.muller
▴ 30
10
votes
36
replies
5.8k
views
Design in DESeq: Can you combine explicit and implicit batch effect correction in (SVA+Metadata Variables)?
SVA
batch-effect
DESeq2
R
updated 6 days ago by
Ram
43k • written 5.7 years ago by
Kristin Muench
▴ 620
10
votes
7
replies
7.1k
views
6 follow
Convert GVF to VCF
vcf
updated 7 days ago by
george-hall-ucl
• 0 • written 9.2 years ago by
win
▴ 970
9
votes
6
replies
1.5k
views
What in silico PCR commandline tool do you use that can handle IUPAC characters?
pcr
amplicon
primer
updated 21 days ago by
Ram
43k • written 16 months ago by
O.rka
▴ 710
9
votes
4
replies
265
views
Ubuntu storage space is decreasing automatically
ubuntu
storage
updated 14 days ago by
colindaven
6.4k • written 14 days ago by
analyst
▴ 20
9
votes
4
replies
1.9k
views
Integration of RNA-Seq data with microarray data
microarray
RNA-Seq
batch-effect
R
updated 6 days ago by
Ram
43k • written 4.0 years ago by
Fahimeh.6868
▴ 30
9
votes
10
replies
4.4k
views
Using rnaseq reference sample across batches
RNA-Seq
batch-effect
updated 6 days ago by
Ram
43k • written 7.4 years ago by
denalitastic
▴ 30
1,163 results • Page
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Comment: Ideal PC configurations and operating system for bioinformatics laboratory
RNA-seq power estimation using ssizeRNA program
Answer: WGCNA preservation analysis
Comment: Ideal PC configurations and operating system for bioinformatics laboratory
ISCN annotation for SV/CN VCF files
Comment: How to convert plink files to Hapmap Format
Am I crazy, or are most published RNA-seq studies vastly underpowered?
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Comment: Ideal PC configurations and operating system for bioinformatics laboratory
by
GenoMax
141k
> What specifications and operating system would be ideal for my case? For choice of OS: Linux to access the large pool of open source sof…
Comment: How to convert plink files to Hapmap Format
by
bk11
★ 2.3k
Ok. First update your phenotypes because in the 6th column all the values are -9 meaning missing, and then run `--assoc`. You can do the fo…
Comment: Ideal PC configurations and operating system for bioinformatics laboratory
by
i.sudbery
19k
You'll probably get away with using one of the other analysis pipelines, like Alevin-fry with much less than 128GB of RAM. Workstations can…
Comment: Difference between samtools "-f 9" and "-f 11"
by
Dylan
• 0
> (which is weird, if it's in proper pair, read and its mate should be both mapped) So that was my thought as well, I think the code you l…
Comment: Ideal PC configurations and operating system for bioinformatics laboratory
by
Ram
43k
> several single cell samples At the same time? You need a server, not a PC. Google around (and search the site as well with keyword "conf…
Answer: Difference between samtools "-f 9" and "-f 11"
by
Pierre Lindenbaum
161k
9: read must be paired AND mate must be unmapped 11: read must be paired AND mapped in proper pair AND mate must be unmapped (which is w…
Comment: How to convert plink files to Hapmap Format
by
Sofia
• 0
the data is binary and associated with a disease but we're studying only the cases and there are other phenotypes of severity
Comment: Cell barcode whitelists for DNBelab C Series High-throughput Single-cell RNA Ser
by
atowns21
• 0
could you share an example command of using STARSolo for the alignment? Did you use the json file directly? Or do you have to re-format the…
Comment: How to trim transcripts using information from NCBI contamination screen report
by
GenoMax
141k
> I didn't do a good job with Trimmomatic for some reason so I have to do some trimming of my transcripts. If you had extraneous sequence…
Comment: How to convert plink files to Hapmap Format
by
bk11
★ 2.3k
Please answer my above question. What type of data is it, binary (cases and controls) or quantitative? You need to update Phenotype info in…
Comment: How to convert plink files to Hapmap Format
by
Sofia
• 0
This is how my .fam file looks like ![enter image description here][1] [1]: /media/images/caccd97c-3606-48d2-aa32-423a9882 And I run …
Comment: How to convert plink files to Hapmap Format
by
bk11
★ 2.3k
What type of phenotype is in your data? It looks like it does not have affection status (default values for affection status will be: 1 = u…
Comment: Redirection of Duplicate PMIDs
by
LauferVA
4.2k
Agree. This is also a great solution that flips the problem on its head, as it were, Dominick. I dont want to be preachy, but this is why i…
Answer: Redirection of Duplicate PMIDs
by
GenoMax
141k
> linked to a list of grants Using [**EntrezDirect**][1] this may be much simpler if you have the grant number available. I tested this w…
Answer: Redirection of Duplicate PMIDs
by
LauferVA
4.2k
Hi Dominick, One thing I am not sure of is, how is it that you got the other PMID in the first place? The reason I ask is that you mentio…
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